HPMSCs protects against D-galactose-induced oxidative damage of CD4⁺ T cells through activating Akt-mediated Nrf2 antioxidant signaling.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) were considered a regenerative therapeutic approach in both acute and chronic diseases. However, whether MSCs regulate the antioxidant metabolism of CD4⁺ T cells and weaken immunosenescence remains unclear. Here, we reported the protective effects of hPMSCs in aging-related CD4⁺ T cell senescence and identified the underlying mechanisms using a D-gal-induced mouse aging model.

Methods

In vivo study, 40 male C57BL/6 mice (8 weeks) were randomly divided into four groups: control group, D-gal group, hPMSC group, and PBS group. In in vitro experiment, human naive CD4⁺ T (CD4CD45RA) cells were prepared using a naive CD4⁺ T cell isolation kit II and pretreated with the Akt inhibitor LY294002 and N

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC7641865 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature