Label-free CARS microscopy reveals similar triacylglycerol acyl chain length and saturation in myocellular lipid droplets of athletes and individuals with type 2 diabetes.

ABSTRACT:

Aims/hypothesis

Intramyocellular lipid (IMCL) content associates with development of insulin resistance, albeit not in insulin-sensitive endurance-trained athletes (trained). Qualitative and spatial differences in muscle lipid composition may underlie this so-called athlete's paradox. Here we studied triacylglycerol (TAG) composition of individual myocellular lipid droplets (LDs) in trained individuals and individuals with type 2 diabetes mellitus.

Methods

Trained ([Formula: see text] 71.0 ± 1.6 ml O₂ [kg lean body mass (LBM)]^-1 min^-1), normoglycaemic (fasting glucose 5.1 ± 0.1 mmol/l) individuals and untrained ([Formula: see text] 36.8 ± 1.5 ml O₂ [kg LBM]^-1 min^-1) individuals with type 2 diabetes (fasting gl

SUBMITTER: Daemen S 

PROVIDER: S-EPMC7641925 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature