A DNA-Launched Nanoparticle Vaccine Elicits CD8⁺ T-cell Immunity to Promote In Vivo Tumor Control.

ABSTRACT: Cytolytic T cells (CTL) play a pivotal role in surveillance against tumors. Induction of CTL responses by vaccination may be challenging, as it requires direct transduction of target cells or special adjuvants to promote cross-presentation. Here, we observed induction of robust CTL responses through electroporation-facilitated, DNA-launched nanoparticle vaccination (DLnano-vaccines). Electroporation was observed to mediate transient tissue apoptosis and macrophage infiltration, which were deemed essential to the induction of CTLs by DLnano-vaccines through a systemic macrophage depletion study. Bolus delivery of protein nano-vaccines followed by electroporation, however, failed to induce CTLs, suggesting direct in vivo production of nano-vaccines may be required. Following these obs

SUBMITTER: Xu Z 

PROVIDER: S-EPMC7642117 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature