Breast Cancer Cell Detection and Characterization from Breast Milk-Derived Cells.

ABSTRACT: Radiologic techniques remain the main method for early detection for breast cancer and are critical to achieve a favorable outcome from cancer. However, more sensitive detection methods to complement radiologic techniques are needed to enhance early detection and treatment strategies. Using our recently established culturing method that allows propagation of normal and cancerous breast epithelial cells of luminal origin, flow cytometry characterization, and genomic sequencing, we show that cancer cells can be detected in breast milk. Cells derived from milk from the breast with cancer were enriched for CD49f⁺/EpCAM^-, CD44⁺/CD24^-, and CD271⁺ cancer stem-like cells (CSC). These CSCs carried mutations within the cytoplasmic retention doma

SUBMITTER: Bhat-Nakshatri P 

PROVIDER: S-EPMC7642166 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature