A Specific and Sensitive Enzymatic Assay for the Quantitation of L-Proline.

ABSTRACT: Because proline accumulates rapidly in response to several stress conditions such as drought and excess salt, increased intracellular levels of free proline are considered a hallmark of adaptive reactions in plants, particularly in response to water stress. Proline quantitation is easily achievable by reaction with ninhydrin, since under acidic conditions peculiar red or yellow reaction products form with this unique cyclic amino acid. However, little attention has been paid to date to cross-reaction of ninhydrin with other amino acids at high levels, or with structurally related compounds that may also be present at significant concentrations in plant tissues, possibly leading to proline overestimation. In vitro at high pH values, δ¹-pyrroline-5-carboxylate reductase, th

SUBMITTER: Forlani G 

PROVIDER: S-EPMC7642206 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature