Piezo1 Forms Specific, Functionally Important Interactions with Phosphoinositides and Cholesterol.

ABSTRACT: Touch, hearing, and blood pressure regulation require mechanically gated ion channels that convert mechanical stimuli into electrical currents. One such channel is Piezo1, which plays a key role in the transduction of mechanical stimuli in humans and is implicated in diseases, such as xerocytosis and lymphatic dysplasia. There is building evidence that suggests Piezo1 can be regulated by the membrane environment, with the activity of the channel determined by the local concentration of lipids, such as cholesterol and phosphoinositides. To better understand the interaction of Piezo1 with its environment, we conduct simulations of the protein in a complex mammalian bilayer containing more than 60 different lipid types together with electrophysiology and mutagenesis experiments. We find that

SUBMITTER: Buyan A 

PROVIDER: S-EPMC7642233 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature