Three-dimensional reconstruction of laryngeal cancer with whole organ serial immunohistochemical sections.

ABSTRACT: Three-dimensional (3D) image reconstruction of tumors based on serial histological sectioning is one of the most powerful methods for accurate high-resolution visualization of tumor structures. However, 3D histological reconstruction of whole tumor has not yet been achieved. We established a high-resolution 3D model of molecular marked whole laryngeal cancer by optimizing the currently available techniques. A series of 5,388 HE stained or immunohistochemically stained whole light microscopic images (200 ×) were acquired (15.61 TB).The data set of block-face images (96.2 GB) was also captured. Direct volume rendering of serial 6.25 × light microscopy images did not demonstrate the major characteristics of the laryngeal cancer as expected. Based on fusion of two datasets, the accurate bounda

SUBMITTER: Tian J 

PROVIDER: S-EPMC7642254 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature