Mapping O₂ concentration in ex-vivo tissue samples on a fast PLIM macro-imager.

ABSTRACT: O₂ PLIM microscopy was employed in various studies, however current platforms have limitations in sensitivity, image acquisition speed, accuracy and general usability. We describe a new PLIM imager based on the Timepix3 camera (Tpx3cam) and its application for imaging of O₂ concentration in various tissue samples stained with a nanoparticle based probe, NanO2-IR. Upon passive staining of mouse brain, lung or intestinal tissue surface with minute quantities of NanO2-IR or by microinjecting the probe into the lumen of small or large intestine fragments, robust phosphorescence intensity and lifetime signals were produced, which allow mapping of O₂ in the tissue within 20 s. Inhibition of tissue respiration or limitation of O₂ diffusion to tissue pro

SUBMITTER: Sen R 

PROVIDER: S-EPMC7642408 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature