Hydrogen Sulfide Protects Against Ammonia-Induced Neurotoxicity Through Activation of Nrf2/ARE Signaling in Astrocytic Model of Hepatic Encephalopathy.

ABSTRACT: Objective: Hepatic encephalopathy (HE) characterized by neuropsychiatric abnormalities is a major complication of cirrhosis with high mortality. However, the pathogenesis of HE has not been fully elucidated. This study aimed to determine endogenous hydrogen sulfide (H₂S) in the blood of HE patients and investigate the role of H₂S in an astrocytic model of HE. Methods: Patients with and without HE were recruited to determine plasma H₂S levels and blood microbial 16S rRNA gene. Rat astrocytes were employed as a model of HE by treatment of NH₄Cl. Exogenous H₂S was preadded. Cell viability was measured by Cell Counting Kit-8 (CCK-8) assay, and cell death was evaluated by lactate dehydrogenase (LDH) release. Apoptosis was dete

SUBMITTER: Jin X 

PROVIDER: S-EPMC7642620 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature