Molecular-replacement phasing using predicted protein structures from <i>AWSEM-Suite</i>.

ABSTRACT: The phase problem in X-ray crystallography arises from the fact that only the intensities, and not the phases, of the diffracting electromagnetic waves are measured directly. Molecular replacement can often estimate the relative phases of reflections starting with those derived from a template structure, which is usually a previously solved structure of a similar protein. The key factor in the success of molecular replacement is finding a good template structure. When no good solved template exists, predicted structures based partially on templates can sometimes be used to generate models for molecular replacement, thereby extending the lower bound of structural and sequence similarity required for successful structure determination. Here, the effectiveness is examined of structures predic

SUBMITTER: Jin S 

PROVIDER: S-EPMC7642774 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature