An automated platform for <i>in situ</i> serial crystallography at room temperature.

ABSTRACT: Direct observation of functional motions in protein structures is highly desirable for understanding how these nanomachineries of life operate at the molecular level. Because cryogenic temperatures are non-physiological and may prohibit or even alter protein structural dynamics, it is necessary to develop robust X-ray diffraction methods that enable routine data collection at room temperature. We recently reported a crystal-on-crystal device to facilitate in situ diffraction of protein crystals at room temperature devoid of any sample manipulation. Here an automated serial crystallography platform based on this crystal-on-crystal technology is presented. A hardware and software prototype has been implemented, and protocols have been established that allow users to image, recognize a

SUBMITTER: Ren Z 

PROVIDER: S-EPMC7642789 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature