Identification and Characterization of Breakpoints and Mutations on Drosophila melanogaster Balancer Chromosomes.

ABSTRACT: Balancers are rearranged chromosomes used in Drosophila melanogaster to maintain deleterious mutations in stable populations, preserve sets of linked genetic elements and construct complex experimental stocks. Here, we assess the phenotypes associated with breakpoint-induced mutations on commonly used third chromosome balancers and show remarkably few deleterious effects. We demonstrate that a breakpoint in p53 causes loss of radiation-induced apoptosis and a breakpoint in Fucosyltransferase A causes loss of fucosylation in nervous and intestinal tissue-the latter study providing new markers for intestinal cell identity and challenging previous conclusions about the regulation of fucosylation. We also describe thousands of potentially harmful mutations shared among

SUBMITTER: Miller DE 

PROVIDER: S-EPMC7642927 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature