New Strains for Tissue-Specific RNAi Studies in Caenorhabditis elegans.

ABSTRACT: RNA interference is a powerful tool for dissecting gene function. In Caenorhabditis elegans, ingestion of double stranded RNA causes strong, systemic knockdown of target genes. Further insight into gene function can be revealed by tissue-specific RNAi techniques. Currently available tissue-specific C. elegans strains rely on rescue of RNAi function in a desired tissue or cell in an otherwise RNAi deficient genetic background. We attempted to assess the contribution of specific tissues to polyunsaturated fatty acid (PUFA) synthesis using currently available tissue-specific RNAi strains. We discovered that rde-1 ( ne219 ), a commonly used RNAi-resistant mutant strain, retains considerable RNAi capacity against RNAi directed at PUFA synthesis genes. B

SUBMITTER: Watts JS 

PROVIDER: S-EPMC7642939 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature