3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography.

ABSTRACT: A multiscale three-dimensional (3D) virtual histology approach is presented, based on two configurations of propagation phase-contrast X-ray tomography, which have been implemented in close proximity at the GINIX endstation at the beamline P10/PETRA III (DESY, Hamburg, Germany). This enables the 3D reconstruction of characteristic morphological features of human pancreatic normal and tumor tissue, as obtained from cancer surgery, first in the form of a large-scale overview by parallel-beam illumination, followed by a zoom into a region-of-interest based on zoom tomography using a Kirkpatrick-Baez mirror with additional waveguide optics. To this end 1 mm punch biopsies of the tissue were taken. In the parallel tomography, a volumetric throughput on the order of 0.01 mm³ s^-1<

SUBMITTER: Frohn J 

PROVIDER: S-EPMC7642968 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature