Rv2577 of <i>Mycobacterium tuberculosis</i> Is a Virulence Factor With Dual Phosphatase and Phosphodiesterase Functions.

ABSTRACT: Tuberculosis, a lung disease caused by Mycobacterium tuberculosis (Mtb), is one of the ten leading causes of death worldwide affecting mainly developing countries. Mtb can persist and survive inside infected cells through modulation of host antibacterial attack, i.e., by avoiding the maturation of phagosome containing mycobacteria to more acidic endosomal compartment. In addition, bacterial phosphatases play a central role in the interplay between host cells and Mtb. In this study, we characterized the Rv2577 of Mtb as a potential alkaline phosphatase/phosphodiesterase enzyme. By an in vitro kinetic assay, we demonstrated that purified Rv2577 expressed in Mycobacterium smegmatis displays both enzyme activities, as evidenced by using the artificial

SUBMITTER: Forrellad MA 

PROVIDER: S-EPMC7642983 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature