Toward Electrophoretic Separation of Membrane Proteins in Supported n-Bilayers.

ABSTRACT: Membrane proteins are key constituents of the proteome of cells but are poorly characterized, mainly because they are difficult to solubilize. Proteome analysis involves separating proteins as a preliminary step toward their characterization. Currently, the most common method is "solubilizing" them with sophisticated detergent and lipid mixtures for later separation via, for instance, sodium dodecyl sulfate polyacrylamide gel electrophoresis. However, this later step induces loss of 3D structure (denaturation). Migration in a medium that mimics the cell membrane should therefore be more appropriate. Here, we present a successful electrophoretic separation of a mixture first of two and then of three different membrane objects in supported n-bilayers. These "objects" are compos

SUBMITTER: Harb FF 

PROVIDER: S-EPMC7643068 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature