Identification and characterization of the proteolytic flagellin from the common freshwater bacterium Hylemonella gracilis.

ABSTRACT: Flagellins are the protein components of bacterial flagella and assemble in up to 20,000 copies to form extracellular flagellar filaments. An unusual family of flagellins was recently discovered that contains a unique metalloprotease domain within its surface-exposed hypervariable region. To date, these proteolytic flagellins (also termed flagellinolysins) have only been characterized in the Gram-positive organism Clostridium haemolyticum, where flagellinolysin was shown to be proteolytically active and capable of cleaving extracellular protein substrates. The biological function of flagellinolysin and its activity in other organisms, however, remain unclear. Here, using molecular biochemistry and proteomics, we have performed an initial characterization of a novel flagellinolysin identifi

SUBMITTER: Eckhard U 

PROVIDER: S-EPMC7643111 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature