Fission yeast condensin contributes to interphase chromatin organization and prevents transcription-coupled DNA damage.

ABSTRACT:

Background

Structural maintenance of chromosomes (SMC) complexes are central organizers of chromatin architecture throughout the cell cycle. The SMC family member condensin is best known for establishing long-range chromatin interactions in mitosis. These compact chromatin and create mechanically stable chromosomes. How condensin contributes to chromatin organization in interphase is less well understood.

Results

Here, we use efficient conditional depletion of fission yeast condensin to determine its contribution to interphase chromatin organization. We deplete condensin in G2-arrested cells to preempt confounding effects from cell cycle progression without condensin. Genome-wide chromatin interaction mapping, using Hi-C, reveals condensin-mediated chromatin interactions in

SUBMITTER: Kakui Y 

PROVIDER: S-EPMC7643427 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature