Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice.

ABSTRACT:

Background

Endometrial regenerative cells (ERCs), a novel type of mesenchymal-like stem cells, were identified as an attractive candidate for immunoregulation and induction of cardiac allograft tolerance. However, the underlying mechanisms of ERCs in immune regulation still remain largely unclear. The present study is designed to determine whether the expression of Galectin-9 (Gal-9), a soluble tandem-repeat member of the galectin family, is crucial for ERC-based immunomodulation.

Methods

In this study, we measured Gal-9 expression on ERCs and then co-cultured Gal-9-ERCs, ERCs, and ERCs+lactose (Gal-9 blocker) with activated C57BL/6-derived splenocytes. Furthermore, we performed mouse heart transplantation between BALB/c (H-2^d) donor and C57BL/6 (H-2^b)

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7643467 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature