Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria.

ABSTRACT:

Background

Bacterial biofilms have become a major threat to human health. The objective of this study was to isolate amylase-producing bacteria from soil to determine the overall inhibition of certain pathogenic bacterial biofilms.

Methods

We used serial dilution and the streaking method to obtain a total of 75 positive amylase isolates. The starch-agar plate method was used to screen the amylolytic activities of these isolates, and we used morphological and biochemical methods to characterize the isolates. Optimal conditions for amylase production and purification using Sephadex G-200 and SDS-PAGE were monitored. We screened these isolates' antagonistic activities and the purified amylase against pathogenic and multi-drug-resistant human bacteria using the agar disk diffusi

SUBMITTER: Elamary R 

PROVIDER: S-EPMC7643558 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature