Effects of Microbiota on the Treatment of Obesity with the Natural Product Celastrol in Rats.

ABSTRACT:

Background

Obesity has become one of the most serious issues threatening the health of humankind, and we conducted this study to examine whether and how celastrol protects against obesity.

Methods

We fed male Sprague-Dawley rats a high-fat diet and administered celastrol to obese rats for 3 weeks. By recording body weight (BW) and other measures, we identified the effective dose of celastrol for obesity treatment. Feces were collected to perform 16S rRNA sequencing, and hypothalami were extracted for transcriptome sequencing. We then treated leptin knockout rats with celastrol and explored the changes in energy metabolism. Male Institute of Cancer Research (ICR) mice were used to test the acute toxicity of celastrol.

Results

We observed that celastrol reduced BW and p

SUBMITTER: Hu W 

PROVIDER: S-EPMC7643605 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature