MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches.

ABSTRACT: Tiny Earth (TE) is a popular international citizen science program aimed at improving public awareness on the growing antimicrobial resistance problem of which MicroMundo Albacete is a Spanish node. With a protocol that is focused on the isolation of antibiotics-producing actinomycetes from soil, 70% of the high school students in MicroMundo Albacete 2020 isolated colonies with antagonistic activity against Gram-positive tester bacteria. However, no activity was found against Gram-negative bacteria. Here, we further adapted the protocol toward a more targeted screening that also enables isolation of antagonistic bacteria against Gram negatives using two different reverse-antibiosis approaches involving a spraying technique or flipping soil sample disks upside down. Exploiting the soil samp

SUBMITTER: Alvarado M 

PROVIDER: S-EPMC7643607 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature