Modelling human hepato-biliary-pancreatic organogenesis from the foregut-midgut boundary.

ABSTRACT: Organogenesis is a complex and interconnected process that is orchestrated by multiple boundary tissue interactions^1-7. However, it remains unclear how individual, neighbouring components coordinate to establish an integral multi-organ structure. Here we report the continuous patterning and dynamic morphogenesis of hepatic, biliary and pancreatic structures, invaginating from a three-dimensional culture of human pluripotent stem cells. The boundary interactions between anterior and posterior gut spheroids differentiated from human pluripotent stem cells enables retinoic acid-dependent emergence of hepato-biliary-pancreatic organ domains specified at the foregut-midgut boundary organoids in the absence of extrinsic factors. Whereas transplant-derived tissues are dominated by midg

SUBMITTER: Koike H 

PROVIDER: S-EPMC7643931 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature