Evaluating riboswitch optimality.

ABSTRACT: Riboswitches are RNA elements that recognize diverse chemical and biomolecular inputs, and transduce this recognition process to genetic, fluorescent, and other engineered outputs using RNA conformational changes. These systems are pervasive in cellular biology and are a promising biotechnology with applications in genetic regulation and biosensing. Here, we derive a simple expression bounding the activation ratio-the proportion of RNA in the active vs. inactive states-for both ON and OFF riboswitches that operate near thermodynamic equilibrium: 1+[I]/K_d^I, where [I] is the input ligand concentration and K_d^I is the intrinsic dissociation constant of the aptamer module toward the input ligand. A survey of published studies of natural and synthetic

SUBMITTER: Wayment-Steele H 

PROVIDER: S-EPMC7644385 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature