Reflection-mode virtual histology using photoacoustic remote sensing microscopy.

ABSTRACT: Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging. These processes are both labor intensive and contribute to creating significant delays in clinical feedback for treatment decisions that can extend to 2-3 weeks for standard paraffin-embedded tissue preparation and interpretation, especially if ancillary testing is needed. Here, we present the first comprehensive study on the broad application of a novel label-free reflection-mode imaging modality known as photoacoustic remote sensing (PARS) for visualizing salient subcellular structures from various common histopathological tissue preparations and for

SUBMITTER: Bell K 

PROVIDER: S-EPMC7644651 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature