A primer set for the rapid isolation of scFv fragments against cell surface antigens from immunised rats.

ABSTRACT: Antibody phage display is a powerful platform for discovery of clinically applicable high affinity monoclonal antibodies against a broad range of targets. Libraries generated from immunized animals offer the advantage of in vivo affinity-maturation of V regions prior to library generation. Despite advantages, few studies have described isolation of antibodies from rats using immune phage display. In our study, we describe a novel primer set, covering the full rat heavy chain variable and kappa light chain variable regions repertoire for the generation of an unbiased immune libraries. Since the immune repertoire of rats is poorly understood, we first performed a deep sequencing analysis of the V(D)J regions of VH and VLK genes, demonstrating the high abundance of IGVH2 and IGVH5 families fo

SUBMITTER: Nannini F 

PROVIDER: S-EPMC7644676 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature