Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor.

ABSTRACT: A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL^-1 and the detection range is 0.1-600 µg.mL^-1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper th

SUBMITTER: Chawjiraphan W 

PROVIDER: S-EPMC7644748 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature