Genetically Encoded Calcium Indicators for In Situ Functional Studies of Corneal Nerves.

ABSTRACT:

Purpose

Millions of people suffer from diseases that involve corneal nerve dysfunction, caused by various conditions, including dry eye syndrome, neurotrophic keratopathy, diabetes, herpes simplex, glaucoma, and Alzheimer's disease. The morphology of corneal nerves has been studied extensively. However, corneal nerve function has only been studied in a limited fashion owing to a lack of tools. Here, we present a new system for studying corneal nerve function.

Methods

Optical imaging was performed on the cornea of excised murine globes taken from a model animal expressing a genetically encoded calcium indicator, GCaMP6f, to record calcium transients. A custom perfusion and imaging chamber for ex vivo murine globes was designed to maintain and stabilize the cornea, while allow

SUBMITTER: McPheeters MT 

PROVIDER: S-EPMC7645214 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature