DNA methylation profiling of CD04⁺/CD08⁺ T cells reveals pathogenic mechanisms in increasing hyperglycemia: PIRAMIDE pilot study.

ABSTRACT:

Background

DNA methylation can play a pathogenic role in the early stages of hyperglycemia linking homeostasis imbalance and vascular damage.

Material and methods

We investigated DNA methylome by RRBS in CD04⁺ and CD08⁺ T cells from healthy subjects (HS) to pre-diabetics (Pre-Diab) and type 2 diabetic (T2D) patients to identify early biomarkers of glucose impairment and vascular damage. Our cross-sectional study enrolled 14 individuals from HS state to increasing hyperglycemia (pilot study, PIRAMIDE trial, NCT03792607).

Results

Globally, differentially methylated regions (DMRs) were mostly annotated to promoter regions. Hypermethylated DMRs were greater than hypomethylated in CD04⁺ T cells whereas CD08⁺ T showed an opposite

SUBMITTER: Benincasa G 

PROVIDER: S-EPMC7645316 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature