Performance evaluation of a high-throughput separation system for circulating tumor cells based on microcavity array.

ABSTRACT: Circulating tumor cells (CTCs) are widely known as useful biomarkers in the liquid biopsies of cancer patients. Although single-cell genetic analysis of CTCs is a promising diagnostic tool that can provide detailed clinical information for precision medicine, the capacity of single-CTC isolation for genetic analysis requires improvement. To overcome this problem, we previously developed a multiple single-cell encapsulation system for CTCs using hydrogel-encapsulation, which allowed for the high-throughput isolation of single CTCs. However, isolation of a single cell from adjacent cells remained difficult and often resulted in contamination by neighboring cells due to the limited resolution of the generated hydrogel. We developed a novel multiple single-cell encapsulation system equipped wi

SUBMITTER: Negishi R 

PROVIDER: S-EPMC7645638 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature