An iron corrosion-assisted H2-supplying system: a culture method for methanogens and acetogens under low H2 pressures.
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ABSTRACT: H2 is an important fermentation intermediate in anaerobic environments. Although H2 occurs at very low partial pressures in the environments, the culture and isolation of H2-utilizing microorganisms is usually carried out under very high H2 pressures, which might have hampered the discovery and understanding of microorganisms adapting to low H2 environments. Here we constructed a culture system designated the "iron corrosion-assisted H2-supplying (iCH) system" by connecting the gas phases of two vials (one for the iron corrosion reaction and the other for culturing microorganisms) to achieve cultures of microorganisms under low H2 pressures. We conducted enrichment cultures for methanogens and acetogens using rice
SUBMITTER: Kato S
PROVIDER: S-EPMC7645788 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
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