Dataset Information
Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China.
Ontology highlight
ABSTRACT: Background
A carbapenem-resistant Escherichia coli (sequence type 5415) strain was isolated from a male patient through routine surveillance in 2018 in Guangzhou, China.Materials and methods
Bacteria were isolated from a sputum culture and identified by using the Vitek 2 compact system. The blaNDM-5 gene was amplified and confirmed by sequencing. Antimicrobial susceptibility testing was determined by a Vitek 2 compact system. The blaNDM-5 gene was located by Southern blotting. Whole-genome sequencing was carried out using both Illumina MiSeq and Oxford Nanopore MinION.Results
S1-PFGE and Southern blotting showed that the bla NDM-5 gene was located on a novel 66-kb IncFII [F2:A-:B-] plasmid. Conjugation assays revealed that the bla NDM-5-bearing plasmid was self-transferrable. Genomic sequencing and comparative analysis suggested that plasmid p2947-NDM5 likely originated from a combination of an IncFII-type backbone and the bla NDM-5 flanking genetic elements.Conclusion
This is the first report of an ST5414 E. coli strain expressing an NDM-5 β-lactamase. This study highlights the genetic complexity of bla NDM-5 carrying plasmids and the urgent need for continuous active monitoring.
SUBMITTER: Yin D
PROVIDER: S-EPMC7646509 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant <i>Escherichia coli</i> Isolate from China.
Infection and drug resistance 20201102
<h4>Background</h4>A carbapenem-resistant <i>Escherichia coli</i> (sequence type 5415) strain was isolated from a male patient through routine surveillance in 2018 in Guangzhou, China.<h4>Materials and methods</h4>Bacteria were isolated from a sputum culture and identified by using the Vitek 2 compact system. The <i>bla<sub>NDM-5</sub></i> gene was amplified and confirmed by sequencing. Antimicrobial susceptibility testing was determined by a Vitek 2 compact system. The <i>bla<sub>NDM-5</sub></i ...[more]