Knockdown of AMPKα2 impairs epithelial‑mesenchymal transition in rat renal tubular epithelial cells by downregulating ETS1 and RPS6KA1.

ABSTRACT: Epithelial‑mesenchymal transition (EMT) serves an important regulatory role in obstructive nephropathy and renal fibrosis. As an intracellular energy sensor, AMP‑activated protein kinase (AMPK) is essential in the process of EMT. The aim of the present study was to elucidate changes in the expression levels of AMPKα2 and which AMPKα2 genes play a role during EMT. TGF‑β1 was used to induce EMT in normal rat renal tubular epithelial (NRK‑52E) cells. The short hairpin AMPKα2 lentivirus was used to interfere with AMPKα2 expression levels in EMT‑derived NRK‑52E cells and AMPKα2 expression levels and EMT were detected. Differential gene expression levels following AMPKα2 knockdown in EMT‑derived NRK‑52E cells were assessed via gene microarray. Potential regulatory pathways were analyzed using in

SUBMITTER: Yin X 

PROVIDER: S-EPMC7646838 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature