Fluocell for Ratiometric and High-Throughput Live-Cell Image Visualization and Quantitation.

ABSTRACT: Spatiotemporal regulation of molecular activities dictates cellular function and fate. Investigation of dynamic molecular activities in live cells often requires the visualization and quantitation of fluorescent ratio image sequences with subcellular resolution and in high throughput. Hence, there is a great need for convenient software tools specifically designed with these capabilities. Here we describe a well-characterized open-source software package, Fluocell, customized to visualize pixelwise ratiometric images and calculate ratio time courses with subcellular resolution and in high throughput. Fluocell also provides group statistics and kinetic analysis functions for the quantified time courses, as well as 3D structure and function visualization for ratio images. The application of

SUBMITTER: Qin Q 

PROVIDER: S-EPMC7646842 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature