MiR‑132 is upregulated in polycystic ovarian syndrome and inhibits granulosa cells viability by targeting Foxa1.

ABSTRACT: Polycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic disorders characterized by hyperandrogenism, polycystic ovaries and ovulatory dysfunction. Several studies have suggested that the aberrant expression of microRNAs (miRNAs/miRs) plays an important role in the pathogenesis of PCOS; however, the role and underlying mechanisms of miR‑132 in the development of PCOS remain unclear. In the present study, the expression of miR‑132 in granulosa cells (GCs) derived from 26 patients with PCOS and 30 healthy controls was detected by reverse transcription‑quantitative PCR (RT‑qPCR). The apoptosis of GCs was examined using a TUNEL assay. The human ovarian granulosa‑like tumor cell line, KGN, was cultured for Cell Counting Kit‑8 assays following the overexpression or knockdow

SUBMITTER: Cui X 

PROVIDER: S-EPMC7646966 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature