Glycogene expression profiles from a HaCaT cell line stably transfected with HPV16 E5 oncogene.

ABSTRACT: The altered expression of glycan antigens has been reported during cervix transformation, demonstrating increased mRNA levels of certain glycogenes. Human papillomavirus (HPV) is the aetiological agent of cervical cancer. High risk HPV E5 is considered an oncogene and has been implicated in cell transformation. E6 and E7 HPV oncoproteins modify the expression of certain glycogenes. The role of the E5 HPV protein in glycogene expression changes has not yet been reported. The aim of the present study was to determine the effects of HPV16 E5 oncoprotein on glycogene expression. For these, a microarray assay was performed using the HaCaT cell line and altered glycogenes were identified. The mRNA levels of certain glycogenes were determined via reverse transcription‑quantitative PCR (RT‑qPCR).

SUBMITTER: Cisneros-Ramirez D 

PROVIDER: S-EPMC7647045 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature