Client proximity enhancement inside cellular membrane-less compartments governed by client-compartment interactions.

ABSTRACT: Membrane-less organelles or compartments are considered to be dynamic reaction centers for spatiotemporal control of diverse cellular processes in eukaryotic cells. Although their formation mechanisms have been steadily elucidated via the classical concept of liquid-liquid phase separation, biomolecular behaviors such as protein interactions inside these liquid compartments have been largely unexplored. Here we report quantitative measurements of changes in protein interactions for the proteins recruited into membrane-less compartments (termed client proteins) in living cells. Under a wide range of phase separation conditions, protein interaction signals were vastly increased only inside compartments, indicating greatly enhanced proximity between recruited client proteins. By employing an

SUBMITTER: Song D 

PROVIDER: S-EPMC7648067 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature