From deterministic to fuzzy decision-making in artificial cells.

ABSTRACT: Building autonomous artificial cells capable of homeostasis requires regulatory networks to gather information and make decisions that take time and cost energy. Decisions based on few molecules may be inaccurate but are cheap and fast. Realizing decision-making with a few molecules in artificial cells has remained a challenge. Here, we show decision-making by a bistable gene network in artificial cells with constant protein turnover. Reducing the number of gene copies from 10⁵ to about 10 per cell revealed a transition from deterministic and slow decision-making to a fuzzy and rapid regime dominated by small-number fluctuations. Gene regulation was observed at lower DNA and protein concentrations than necessary in equilibrium, suggesting rate enhancement by co-expressional loca

SUBMITTER: Greiss F 

PROVIDER: S-EPMC7648101 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature