Pharmacological validation of targets regulating CD14 during macrophage differentiation.

ABSTRACT: The signalling receptor for LPS, CD14, is a key marker of, and facilitator for, pro-inflammatory macrophage function. Pro-inflammatory macrophage differentiation remains a process facilitating a broad array of disease pathologies, and has recently emerged as a potential target against cytokine storm in COVID19. Here, we perform a whole-genome CRISPR screen to identify essential nodes regulating CD14 expression in myeloid cells, using the differentiation of THP-1 cells as a starting point. This strategy uncovers many known pathways required for CD14 expression and regulating macrophage differentiation while additionally providing a list of novel targets either promoting or limiting this process. To speed translation of these results, we have then taken the approach of independently validati

SUBMITTER: Jimenez-Duran G 

PROVIDER: S-EPMC7648121 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature