Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2.

ABSTRACT: In late 2019, an outbreak of respiratory disease named COVID-19 started in the world. To date, thousands of cases of infection are reported worldwide. Most researchers focused on epidemiology and clinical features of COVID-19, and a small part of studies was performed to evaluate the genetic characteristics of this virus. Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Using bioinformatics databases and software, we designed the PCR-RFLP method for D614G mutation detection. We evaluated 144 SARS-CoV-2 positive samples isolated in six months in Northeastern Iran. Our results showed that the prevalent type is S-D in our isolates, and a small numbe

SUBMITTER: Hashemi SA 

PROVIDER: S-EPMC7648195 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature