Validated Reference Panel from Renewable Source of Genomic DNA Available for Standardization of Blood Group Genotyping.

ABSTRACT: Extended blood group genotyping is an invaluable tool used for prevention of alloimmunization. Genotyping is particularly suitable when antigens are weak, specific antisera are unavailable, or accurate phenotyping is problematic because of a disease state or recent transfusions. In addition, genotyping facilitates establishment of mass-scale patient-matched donor databases. However, standardization of genotyping technologies has been hindered by the lack of reference panels. A well-characterized renewable reference panel for standardization of blood group genotyping was developed. The panel consists of genomic DNA lyophilized and stored in glass vials. Genomic DNA was extracted in bulk from immortalized lymphoblastoid cell lines, generated by Epstein-Barr virus transformation of peripheral

SUBMITTER: Volkova E 

PROVIDER: S-EPMC7648275 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature