High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method.

ABSTRACT:

Background

Genome-centric approaches are widely used to investigate microbial compositions, dynamics, ecology, and interactions within various environmental systems. Hundreds or even thousands of genomes could be retrieved in a single study contributed by the cost-effective short-read sequencing and developed assembly/binning pipelines. However, conventional binning methods usually yield highly fragmented draft genomes that limit our ability to comprehensively understand these microbial communities. Thus, to leverage advantage of both the long and short reads to retrieve more complete genomes from environmental samples is a must-do task to move this direction forward.

Results

Here, we used an iterative hybrid assembly (IHA) approach to reconstruct 49 metagenome-assembled gen

SUBMITTER: Liu L 

PROVIDER: S-EPMC7648391 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature