Skeletal muscle healing by M1-like macrophages produced by transient expression of exogenous GM-CSF.

ABSTRACT:

Background

After traumatic skeletal muscle injury, muscle healing is often incomplete and produces extensive fibrosis. The sequence of M1 and M2 macrophage accumulation and the duration of each subtype in the injured area may help to direct the relative extent of fibrogenesis and myogenesis during healing. We hypothesized that increasing the number of M1 macrophages early after traumatic muscle injury would produce more cellular and molecular substrates for myogenesis and fewer substrates for fibrosis, leading to better muscle healing.

Methods

To test this hypothesis, we transfected skeletal muscle with a plasmid vector to transiently express GM-CSF shortly after injury to drive the polarization of macrophages towards the M1 subset. C57BL/6 mouse tibialis anterior (TA) muscl

SUBMITTER: Martins L 

PROVIDER: S-EPMC7648431 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature