Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium.

ABSTRACT: The genetic regulatory network controlling early fate choices during human blood cell development are not well understood. We used human pluripotent stem cell reporter lines to track the development of endothelial and haematopoietic populations in an in vitro model of human yolk-sac development. We identified SOX17^-CD34⁺CD43^- endothelial cells at day 2 of blast colony development, as a haemangioblast-like branch point from which SOX17^-CD34⁺CD43⁺ blood cells and SOX17⁺CD34⁺CD43^- endothelium subsequently arose. Most human blood cell development was dependent on RUNX1. Deletion of RUNX1 only permitted a single wave of yolk sac-like primitive erythropoiesis, but no yolk sac myelopoi

SUBMITTER: Bruveris FF 

PROVIDER: S-EPMC7648599 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature