An HIV-Tat inducible mouse model system of childhood HIV-associated nephropathy.

ABSTRACT: Modern antiretroviral therapies (ART) have decreased the prevalence of HIV-associated nephropathy (HIVAN). Nonetheless, we continue to see children and adolescents with HIVAN all over the world. Furthermore, once HIVAN is established in children, it is difficult to revert its long-term progression, and we need better animal models of childhood HIVAN to test new treatments. To define whether the HIV-1 trans-activator (Tat) gene precipitates HIVAN in young mice, and to develop an inducible mouse model of childhood HIVAN, an HIV-Tat gene cloned from a child with HIVAN was used to generate recombinant adenoviral vectors (rAd-Tat). rAd-Tat and LacZ control vectors (2×10⁹) were expressed in the kidney of newborn wild-type and HIV-transgenic (Tg₂₆

SUBMITTER: Tang P 

PROVIDER: S-EPMC7648609 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature