MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks.

ABSTRACT: Brucellosis is one of the most common bacterial zoonoses worldwide affecting not only livestock and wildlife but also pets. Canine brucellosis is characterized by reproductive failure in dogs. Human Brucella canis infections are rarely reported but probably underestimated due to insufficient diagnostic surveillance. To improve diagnostics, we investigated dogs in a breeding kennel that showed clinical manifestations of brucellosis and revealed positive blood cultures. As an alternative to the time-consuming and hazardous classical identification procedures, a newly developed species-specific intact-cell matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis was applied, which allowed for rapid identification of B. canis and differentiation from closely relate

SUBMITTER: da Silva DAV 

PROVIDER: S-EPMC7648634 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature