Multimodal Single-Cell Analysis Reveals Physiological Maturation in the Developing Human Neocortex.

ABSTRACT: In the developing human neocortex, progenitor cells generate diverse cell types prenatally. Progenitor cells and newborn neurons respond to signaling cues, including neurotransmitters. While single-cell RNA sequencing has revealed cellular diversity, physiological heterogeneity has yet to be mapped onto these developing and diverse cell types. By combining measurements of intracellular Ca²⁺ elevations in response to neurotransmitter receptor agonists and RNA sequencing of the same single cells, we show that Ca²⁺ responses are cell-type-specific and change dynamically with lineage progression. Physiological response properties predict molecular cell identity and additionally reveal diversity not captured by single-cell transcriptomics. We find that the serotonin recept

SUBMITTER: Mayer S 

PROVIDER: S-EPMC7648658 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature