Quantitative methods in microscopy to assess pollen viability in different plant taxa.

ABSTRACT: High-quality pollen is a prerequisite for plant reproductive success. Pollen viability and sterility can be routinely assessed using common stains and manual microscope examination, but with low overall statistical power. Current automated methods are primarily directed towards the analysis of pollen sterility, and high throughput solutions for both pollen viability and sterility evaluation are needed that will be consistent with emerging biotechnological strategies for crop improvement. Our goal is to refine established labelling procedures for pollen, based on the combination of fluorescein (FDA) and propidium iodide (PI), and to develop automated solutions for accurately assessing pollen grain images and classifying them for quality. We used open-source software programs (CellProfiler,

SUBMITTER: Ascari L 

PROVIDER: S-EPMC7648740 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature