Phylogenetic analysis of Trichostrongylus vitrinus isolates from southwest Iran.

ABSTRACT:

Background

Trichostrongylus is one of the most important zoonotic trichostrongylid nematodes, infecting mostly livestock. Data on its genetic characteristics are lacking in Iran.

Methods

We determined the phylogenetic relationships of Trichostrongylus species in three counties of Kohgiloyeh and Boyerahmad (K-B) province, southwest Iran. Small intestine and abomasum of 70 sheep and goats were investigated.

Results

A total of 35 isolates of Trichostrongylus worms were detected and all were genetically identified as Trichostrongylus vitrinus. Analysis of 321 bp of the internal transcribed spacer 2 (ITS2) of ribosomal DNA revealed 16 genotypes. All genotypes were single nucleotide polymorphisms, including some hypervariable points. All sequences were trimmed to 170 bp, co

SUBMITTER: Ghatee MA 

PROVIDER: S-EPMC7648934 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature