Development of a qPCR for the detection and quantification of Salmonella spp. in sheep feces and tissues.

ABSTRACT: Salmonella spp. are common causes of disease in intensive livestock production systems, and contamination of foodstuffs is of significant concern for public health. Therefore, the identification and quantification of Salmonella spp. is important for monitoring the level of fecal shedding or tissue colonization in infected animals and animal products. We developed and evaluated a quantitative PCR (qPCR) method on spiked sheep tissue and fecal samples for the detection and quantification of Salmonella spp. Without the use of a pre-enrichment step, the qPCR limit of detection (LOD) results for sheep fecal (4 × 10⁴-6 × 10³ cfu/g) and tissue (4 × 10⁵-4 × 10³ cfu/g) samples were not adequate for detection purposes. With the inclusi

SUBMITTER: Parker AM 

PROVIDER: S-EPMC7649550 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature